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plenti ef1a backbone ng  (Addgene inc)


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    Addgene inc plenti ef1a backbone ng
    Plenti Ef1a Backbone Ng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+ef1a+backbone+plasmids/pLenti-EF1a-Backbone(NG)+(Plasmid+%2327963)/pmc11303488-54-26-28
    Average 85 stars, based on 3 article reviews
    plenti ef1a backbone ng - by Bioz Stars, 2026-09
    85/100 stars

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    Expressing:

    Article Title: A Simple and Efficient Method for Assembling TALE Protein Based on Plasmid Library
    Article Snippet: Taq and Pfu DNA polymerase were bought from Transgen Biotech (Beijing, China). pGEM-T easy vector was bought from Tiangen Biotech (Beijing, China). .. 4 monomer fragments (NI, HD, NG and NN), 4 pLenti-EF1a-Backbone plasmids (backbone plasmids for TALE-TF construction and expression) and pST1374 vector (backbone plasmids for TALEN construction and expression) were bought from Addgene (USA). .. Plasmid preparations were performed by using Plasmid Mini Kit (Omega, USA) and DNA gel extractions by using AxyPrepTM DNA Gel Extraction Kit (Axygen, USA) following the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: A Simple and Efficient Method for Assembling TALE Protein Based on Plasmid Library
    Article Snippet: Taq and Pfu DNA polymerase were bought from Transgen Biotech (Beijing, China). pGEM-T easy vector was bought from Tiangen Biotech (Beijing, China). .. 4 monomer fragments (NI, HD, NG and NN), 4 pLenti-EF1a-Backbone plasmids (backbone plasmids for TALE-TF construction and expression) and pST1374 vector (backbone plasmids for TALEN construction and expression) were bought from Addgene (USA). .. Plasmid preparations were performed by using Plasmid Mini Kit (Omega, USA) and DNA gel extractions by using AxyPrepTM DNA Gel Extraction Kit (Axygen, USA) following the manufacturer’s protocol.



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    Image Search Results


    Journal: eLife

    Article Title: SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B

    doi: 10.7554/eLife.67452

    Figure Lengend Snippet:

    Article Snippet: Transfected construct ( M. musculus ) , pLenti-III-ef1α (plasmid) , Addgene (a gift from ) , http://n2t.net/addgene : 27964; RRID: Addgene_27964 , Backbone vector of Sirt1WT and HY mutant, Smpdl3b and H135A mutant expression constructs.

    Techniques: Generated, CRISPR, Plasmid Preparation, Stable Transfection, Expressing, Transfection, Construct, Clone Assay, shRNA, Mutagenesis, Recombinant, Luciferase, Sequencing, In Vitro, Staining, Isolation, SYBR Green Assay, Real-time Polymerase Chain Reaction, Labeling, Northern Blot, Hybridization, Purification, Reporter Assay, Activity Assay, Software